αCHCA matrix kit

50 eppendorf tubes of recrystallized α-cyano-4-hydroxycinnamic acid (αCHCA) matrix (5 mg each) and one 50 mL solvent vial (50% acetonitrile in water / 0.1% Trifluoroacetic acid) for matrix preparation.


Applications: MALDI-TOF analysis of peptides and proteins, Proteomics

SA matrix kit

50 eppendorf tubes of recyrstallized Sinapinic acid matrix (3,5 dimethoxy-4-hydroxycinnamic acid / SA - 5 mg each), and one 50 mL solvent vial (30% acetonitrile in water / 0.1% trifluoroacetic acid) for matrix preparation.

Applications: MALDI-TOF analysis of peptides and proteins

DHB matrix kit

50 eppendorf tubes of recrystalllized 2,5 dihydroxybenzoic acid matrix (Gentisic acid - DHB - 5 mg each), and one 50 mL solvent vial (20% acetonitrile in water / 0.1% trifluoroacetic acid) for matrix preparation.

Applications: MALDI-TOF analysis of peptides and proteins, small molecules

Peptide/protein 3-matrix kit

- 30 eppendorf tubes of recrystallized α-cyano-4-hydroxycinnamic acid matrix (αCHCA, 5 mg each)
- 30 eppendorf tubes of recrystallized Sinapinic acid (3,5 dimethoxy-4-hydroxycinnamic acid matrix / SA - 5 mg each)
- 30 eppendorf tubes of recrystallized 2,5 dihydroxybenzoic acid matrix (Gentisic acid / DHB - 5 mg each)
- Three solvent vials of 30 mL each (50% acetonitrile in water / 0.1% TFA, 30% acetonitrile in water / 0.1% TFA, 20% acetonitrile in water / 0.1% TFA) for matrix preparation


Applications: MALD-TOF analysis of peptides and proteins, proteomics, small molecules

sDHB (super DHB) matrix kit

50 eppendorf tubes of recrystallized super DHB matrix (2,5 dihydroxybenzoic acid + 5-methoxysalicylic acid - 5 mg each) and one 50mL solvent vial (20% acetonitrile in water / 0.1% trifluoroacetic) for matrix preparation.

Applications: MALDI-TOF analysis of peptides and proteins

CA matrix kit

50 eppendorf tubes of recrystallized Caffeic acid matrix (3,4 dihydroxycinnamic acid / CA - 5 mg each) and one 50 mL solvent vial (50% acetonitrile in water / 0.1% trifluoroacetic acid) for matrix preparation.

Applications: MALDI-TOF analysis of peptides and proteins

FA matrix kit

50 eppendorf tubes of recrystallized Ferulic acid matrix (trans-4-hyrodxy-3-methoxycinnamic acid / FA - 5 mg each) and one 50 mL solvent vial (30% acetonitrile in water / 0.1% trifluoroacetic acid) for matrix preparation

Applications: MALDI-TOF analysis of peptides and proteins

Peptide/protein 5-matrix kit

- 30 eppendorf tubes of recrystallized α-cyano-4-hydroxycinnamic acid matrix (CHCA - 5 mg each)
- 30 eppendorf tubes of recrystallized Sinapinic acid (3,5 diemthoxy-4-hydroxycinnamic acid matrix / SA - 5 mg each)
- 30 eppendorf tubes of recrystallized 2,5 dihydroxybenzoic acid matrix (Gentistic acid / DHB - 5mg each)
- 30 eppendorf tubes of recrystallized Ferulic acid matrix (trans-4-hydroxy-3-methoxycinnamic acid / FA - 5 mg each)
- Three solvent vials (50% acetonitrile in water / 0.1% TFA, 30% acetonitrile in water / 0.1% TFA, 20% acetonitrile in water / 0.1% TFA) for matrix preparation


Applications: MALDI-TOF analysis of peptides and proteins, proteomics, small molecules

HPA matrix kit

50 eppendorf tubes of recrystallized 3-hydroxypicolinic acid matrix (HPA - 25 mg each), and one 50 mL solvent vial (25mM diammonium citrate in 50% acetonitrile / water / 0.1% TFA) for matrix preparation

Applications: MALDI-TOF analysis of oligonucleotides

246THAP matrix kit

50 eppendorf tubes of recrystallized 2,4,6 trihydroxyacetophenone matrix (246THAP - 10 mg each), and one 50 mL solvent vial (25mM diammonium citrate in 50% acetonitrile / water / 0.1% TFA) for matrix preparation

Applications: MALDI-TOF analysis of oligonucleotides

Ouligonucleotide matrix kit

- 30 eppendorf tubes of recrystallized 3-hydroxypicolinic acid matrix (HPA - 25 mg each)
- 30 eppendorf tubes of recrystallized 2,4,6 trihydroxyacetophenone matrix (246THAP - 10 mg each)
- One 60 mL solvent vial (25mM diammonium citrate in 50% acetonitrile / water / 0.1% TFA) for matrix preparation

Applications: MALDI-TOF analysis of oligonucleotides

Ouligonucleotide matrix kit

- 30 eppendorf tubes of recrystallized 3-hydroxypicolinic acid matrix (HPA - 25 mg each)
- 30 eppendorf tubes of recrystallized 2,4,6 trihydroxyacetophenone matrix (246THAP - 10 mg each)
- One 60 mL solvent vial (25mM diammonium citrate in 50% acetonitrile / water / 0.1% TFA) for matrix preparation

Applications: MALDI-TOF analysis of oligonucleotides

Recrystallized αCHCA matrix

5 tubes of 200 mg each recrystallized α-cyano-4-hydroxycinnamic acid matrix (αCHCA)

Recrystallized SA matrix

5 tubes of 200 mg each recrystallized Sinnapinic acid matrix (3,5 dimethoxy-4-hydroxycinnamic acid / SA)

Recrysallized DHB matrix

5 tubes of 200 each recrystallized 2,5 dihydroxybenzoic acid matrix (Gentisic acid / DHB)

Recrystallized Peptide/protein 3-matrix kit

3 tubes of 200 mg each of recrystallized matrix:
- α-cyano-4-hyroxycinnamic acid (αCHCA)
- Sinapinic acid (3,5 dimethoxy-4-hydroxycinnamic acid - SA)
- 2,5 dihydroxybenzoic acid ( Gentisic acid - DHB)

Recrystallized sDHB matrix

5 tubes of 200 mg each recrystallized super DHB matrix (2,5 dihydroxybenzoic acid + 5-methoxysalicylic acid)

Recrystallized CA matrix

5 tubes of 200 mg each recrystallized Caffeic acid matrix (3,4 dihydroxycinnamic acid / CA)

Recrystallized FA matrix

5 tubes of 200 mg each recrystallized Ferulic acid matrix (trans-4-hydroxy-3-methoxycinnamic acid / FA)

Recrystallized Peptide/protein 5-matrix kit

5 tubes of 200 mg each of recrystallized matrix:
- α-cyano-4-hydroxycinnamic acid (αCHCA)
- Sinapinic acid (3,5 dimethoxy-4-hydroxycinnamic acid - SA)
- 2,5 dihydroxybenzoic acid (Gentistic acid - DHB)
- Caffeic acid matrix (3,4 dihydroxycinnamic acid / CA)
- Ferulic acid matrix (trans-4-hydroxy-3-methoxycinnamic acid / FA)

Recrystallized HPA matrix

5 tubes of 200 each recrystallized 3-hydroxypicolinic acid (HPA)

Recrystallized 246THAP matrix

5 tubes of 200 mg each recrystallized 2,4,6 trihydroxyacetophenone matrix (246THAP)

Recrystallized oligonucleotide 3-matrix kit

3 tubes of 200 mg each recrystallized matrix
- 3-hydroxypicolinic acid matrix (HPA)
- Picolinic acid matrix (PA)
- 2,4,6 trihydroxyacetophenone matrix (246THAP)

Peptide calibration mix 1 (PepMix1) 1000-2500 Da

5 tubes of peptide calibration mixture 1. Each tube provides 1000 calibration points

Contains:

Angiotensi II	(1046.2 Da)
Angiotensi I	(1296.5 Da)
Neurotensin	(1672.9 Da)
ACTH[1-17]	(2093.5 Da)
ACTH[18-39]	(2465.7 Da)

Peptide calibration mix 2 (PepMix2) 1500-6000 Da

5 tubes of peptide calibration mixture 2. Each tube provides 1000 calibration points

Contains:

Neurotensin	(1672.9 Da)
ACTH[18-39]	(2465.7 Da)
Bovine Insulin chain B	(3495.9 Da)
Bovine insulinn	(5733.6 Da)

Peptide calibration mix 3 (PepMix3) 3000-9000 Da

5 tubes of peptide calibration mixture 3. Each tube provides 1000 calibration points

Contains:

Bovine Insulin chain B	(3495.9 Da)
Bovine Insuline	(5733.6 Da)
Aprotinin	(6511.5 Da)
Ubliquitin bovine	(8564.8 Da)

Peptide calibration mix 4 (ProteoMix) 500-3500 Da

5 tubes of peptide calibration mixture 4. Each tube provides 1000 calibration points. Specially developed for calibration of proteolytic peptide mixtures and proteomics applications

Contains:

Bradykinin[1-5]	(572.7 Da)
Angiotensin II	(1046.2 Da)
Neurotensin	(1672.9 Da)
ACTH[18-39]	(2465.7 Da)
Bovine Insulin chain B	(3495.9 Da)

Peptide calibration mix 5 (PepMix5) 500-2000 Da

5 tubes of peptide calibration mixture 5. Each tube provides 1000 calibration points

Contains:

Bradykinin[1-5]	(572.7 Da)
Bradykinin[1-7]	(756.9 Da)
Bradykinin	(1060.2 Da)
Angiotensin I	(1296.5 Da)
Neurotensin	(1672.9 Da)

Peptide calibration mix 6 (PepMix6) 350-1000 Da

5 tubes of peptide calibration mixture 6. Each tube provides 1000 calibration points

Contains:

Bradykinin[1-5]	(572.7 Da)
Bradykinin[1-7]	(756.0 Da)
Bradykinin[1-8]	(904.0 Da)

Protein calibration mix 1 (ProMix1) 5,700-17,0000 Da

5 tubes of protein calibration mixture 1. Each tube provides 1000 calibration points

Contains:

Bovine Insulin	(5733.6 Da)
Horse heart cytochrome C	(12360.1 Da)
Horse myoglobin	(16951.5 Da)

Protein calibration mix 2 (ProMix2) 8,000-24,000 Da

5 tubes of protein calibration mixture 2. Each tube provides 1000 calibration points

Contains:

Horse heart cytochrome C	(12360.1 Da)
Horse myoglobin	(16951.5 Da)
Trypsinogen	(23980.9 Da)

Protein calibration mix 3 (ProMix3) 23,000-66,000 Da

5 tubes of protein calibration mixture 3. Each tube provides 1000 calibration points

Contains:

Trypsinogen	(23980.9 Da)
Yeast enolase	(46670.9 Da)
Bovine serum albumin	(66429.9 Da)

Protein calibration standards 12,000-66,000 Da

5 tubes of individual protein calibrants. Each tube provides 1000 calibration points

Contains:

Horse heart cytochrome C	(12360.1 Da)
Horse myoglobin	(16951.5 Da)
Trypsinogen	(23980.9 Da)
Yeast enolase	(46670.9 Da)
Bovine serum albumin	(66429.9 Da)

Peptide calibration kit I (1000-8500 Da)

- Peptide calibration mixtures 1 (PepMix1), 2 (PepMix2) and 3 (PepMix3). 1 tube each. Each tube provides 1000 calibration points
- Four single calibrant peptides: Angiotensin II (1046.2 Da), ACTH[18-39] (2465.7 Da), Bovine Insulin chain B (3495.9 Da), Bovine Insulin (5733.6 Da)
- 1 tube of mixture diluent (0.01% TFA)

Peptide calibration kit II (0-2000 Da)

- Peptide calibration mixtures 1 (PepMix1), 5 (PepMix5) and 6 (PepMix6). 1 tube each. Each tube provides 1000 calibration points
- Four single calibrant peptides: Bradykinin[1-5] (572.7 Da), Angiotensin II (1046.2 Da), Neurotensin (1672.9 Da), ACTH[18-39] (2465.7 Da)
- 1 tube of mixture diluent (0.01% TFA)

Protein calibration kit (5-100kDa)

- 1 tube each of protein calibration mixtures 1 (ProMix1), 2 (ProMix2) and 3 (ProMix3). Each tube provides 1000 calibration points.
- Four single calibrant proteins: Bovine Insulin (5733.6 Da), Horse myoglobin (16951.5 Da), Yeast enolase (46670.9 Da), Bovine serum albumin (66429.9 Da)
- 1 tube of mixture diluent (0.01% TFA)

Peptide / Protein calibration kit (1-100 kDa)

- 1 tube of peptide calibration mixtures 1 (PepMix1), 2 (PepMix2) and 3 (PepMix3). Each tube provides 1000 calibration points
- 1 tube each of protein calibration mixtures 2 (ProMix2) and 3 (ProMix3). Each tube provides 100 calibration points
- Four single calibrant peptides: Angiotensin II (1046.2 Da), ACTH[18-39] (2465.7 Da), Bovine Insulinn chain B (3495.9 Da), Bovine Insulin (5733.6 Da)
- Four single calibrant proteins: Horse myoglobin (16951.5 Da), Trypsinogen (23980.9 Da), Yeast enolase (46670.9 Da), Bovine serum albumin (66429.9 Da)
- 1 tube of mixture diluent (0.01% TFA)

Myoglobin trypsin digest

5 tubes of digest D101. Each tube contains 50 picomoles of digested protein

Enolase trypsin digest

5 tubes of digest D102. Each tube contains 50 picomoles of digested protein

Glutamate dehydrogenase trypsin digest

5 tubes of digest D103. Each tube contains 50 picomoles of digested protein

BSA trypsin digest

5 tubes of digest D104 Each tube contains 50 picomoles of digested protein

Glycogen phosphorylase trypsin digest

5 tubes of digest D105 Each tube contains 50 picomoles of digested protein

Trypsin digest mix

1 tube each of:
- D101 (Myoglobin)
- D102 (Enolase)
- D103 (Glutamate dehydrogenase)
- D104 (BSA)
- D105 (Glycogen phosphorylase)

Each tube contains 50 picomoles of digested proteins

MALDI imaging starter kit product description

Kit IMG-001 is designed for MALDI imaging applications with the Applied Biosystems / Sciex 4800 and 5800 MALDI TOF/TOF systems. The kit contains a specially designed microscope slide adaptor based on an Opti-TOF® LC-MALDI insert. The insert holds two microscope slides for MALDI imaging experiments.

In order to facilitate the setup of imaging experiments and provide a complete solution, the kit also contains the following components:

- 25 ITO-coated conductive microscope slides. LaserBio Labs offers ITO slides marked on the conductive side for easier handling

- 25 pieces of water-sensitive paper for spray control. The water-sensitive paper is coated with a chemical that turns blue when sprayed with liquid. The papers are the size of miscroscope slides and will allow for optimization of matrix spray conditions.

- 25 pieces of double-side adhesive conductive tape. The tape is used to ensure electrical contact of the microscope slides with the adapter and to mount tissue or material without glass slides.

- 50 pieces of double-sided adhesive to affix the microscope slides in Opti-TOF® holder

- A Matrix & Calibrants MALDI Imaging starter pack. The pack includes peptides and protein mixes for calibration of samples in the small molecule, peptide and protein mass ranges. MALDI matrix (CHCA and SA) is also included for method development

- A kit brochure